Fig. 1. Click the button in the red box to launch Prophage Hunter.
Fig. 2. Click “Browse” to upload FASTA file.
Fig. 3. Enter email address to receive the analysis report.
Fig. 4a. Enter email address to receive the analysis report.
Fig. 4b. User Experience Improvement Program.
Fig. 5. Click “START HUNTING” to search for active prophages.
Fig. 6. Wait until your analysis is completed.
Fig. 7. A genome browser showing locus and category of each predicted prophage region.
- Candidate ID: ID of the predicted prophage region;
- Sequence ID: ID of the genomic sequence shown in the input file;
- Start: start position of the predicted prophage region;
- End: end position of the predicted prophage region;
- Length: length of the predicted prophage region;
- Category: the category of the predicted prophage region;
- Score: score of the predicted prophage region, the higher the score is, the stronger the evidence that supports an active prophage region;
- Closest phage: the closest phage of the predicted prophage region compared to known phages, “N/A” represents not homologous to known phages;
- Gene number: number of predicted prophage genes within the predicted prophage region.
Fig. 8. Details of each predicted prophage region.Users can click the corresponding buttons to download genomic DNA sequences of each region, or CDS of predicted prophage genes, or amino acids sequences of predicted prophage genes (Fig. 8b), or all analysis results (Fig. 8c). Users can also click the taxonomy name of the closest phage to see the details (Fig. 8d).
Fig. 9a. NCBI_nr, Pfam and InterPro annotation of each predicted prophage gene within this region.
Fig. 9b. Top 5 closest phages that homologous to this region.